Allergen Testing in Homes, Offices and Commercial Buildings

Dust Mites

dust mites

Dust mites are ubiquitous and are found in every household. Mites prefer warm, moist surroundings such as the inside of pillows or mattresses. The mites feed on human dander (skin scales) that accumulate in bedding and house dust. Dust mite allergens are proteins that come from the digestive tract of mites and are found in high levels in mite feces. The allergen containing fecal balls are relatively large (~10-20 um) and remain in the air for short periods. Most exposure occurs through disturbance of dust near the breathing zone.

Cats and Dog Allergens

Cats and dogs are the most common animal cohabitants, present in more than 1/3 of homes in the United States. Those who touch cats and dogs or visit households with cats and dogs easily carry these allergens from home to home, office, school, etc. These pet allergens are carried on particles ranging from 1 to 20 mm in diameter and the smaller of these particles can remain airborne for long periods of time. Consequently, pet allergens are spread easily throughout a house even when pets are kept out of certain rooms. Cat and dog allergens are also very sticky and can be found in high levels on walls and other surfaces within homes. Carpeting, bedding, and upholstered furniture can be reservoirs for deposited pet allergens. In most of the studies, a vast majority of homes have been found to contain pet allergens, even if pets are not present. This widespread distribution has been presumed to occur primarily through the passive transfer of these allergens from one environment to another. The major cat and dog allergens are low molecular weight proteins found primarily in animal secretions. They are produced in the sebaceous, salivary, and anal glands. Touching the pet and subsequently transferring allergen from hand to nose is only one mode of contact. The most important route of exposure is by inhalation of airborne allergen. This allows deposition of large quantities of the allergen in both the upper and lower airways.

Example of Guideline Criteria for Cat Allergens in Bulk Dust Sample

Low < 1 μg/g dust
Significant -8 μg/g dust
High > 8 μg/g dust
Note: µg/g = microgram per gram of dust

Cockroach Allergens

Cockroaches are an important source of indoor allergens worldwide. Cockroach allergens are widely distributed in homes and schools and can be found in beds, furniture, and carpets, with the highest levels typically found in the kitchen. However, cockroach allergens may be more relevant in the bedroom than the kitchen or the living room because of close contact with the pillow while in bed.


About 20% of homes with no evidence of cockroach infestation have significant levels of cockroach allergen in settled dust. The level of cockroach allergen in school dust is of concern because it may constitute an occupational risk to students, teachers and other school workers. The sources of cockroach allergens include the gastrointestinal tract, saliva, feces and body parts of the cockroach. As cockroaches die in a dwelling, their decomposing body parts become part of the environmental dust. These sources contain cockroach allergens.

Rodent Allergens

Rodents (mice and rats) can occur in both home and work environments. Exposure to rodents can come either from keeping them as pets or from their presence as pests in the home. Veterinarians, laboratory technicians, etc., can become allergic to rodents due to intensive exposure to these animals in their daily work. Rodents can also be a problem in schools. The sources of rodent allergen include the rodent urine and skin flakes. The rodent’s urine has a high concentration of protein, which is the primary allergen to humans. The urine is often sprayed rather than deposited, thereby increasing human exposure. After the urine dries, the urinary proteins become airborne and are inhaled by humans, leading to allergic symptoms.

Allergen samples are typically collected by micro-vacuuming a sample of bulk dust into a cassette and having the collected dust analyzed. There are guidelines for the common types of allergens described above.

All testing is performed by Certified Industrial Hygienists with a minimum of 20 years experience. Samples are analyzed at an accredited laboratory using ELISA method.

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